College of Science

SEMINAR: Ertan Salik [Cal Poly Pomona]

From label-free fiber optic biosensors to fluorescent label based bioimaging

Apr 24, 2014 10:50 AM to Apr 24, 2014 12:00 PM at Building 8, Room 241

Ertan SalikBiosensors find numerous uses in medical diagnosis, food safety, environmental protection, and biodefense. In the first part of my seminar, I will summarize our development of tapered fiber optic biosensors. We have demonstrated specific detection of < 500 ng/mL proteins using their antibodies. Recently, we have shown that tapered fiber sensors can be made extremely sensitive by optimizing their waist diameter. Experimentally, we have demonstrated a refractive index sensitivity of nearly 10000 nm/RIU, an order of magnitude higher than the typical published results. I will also summarize our efforts to embed fiber optic sensors within microfluidic channels to improve their robustness.

In the second part of the seminar, I will talk about fluorescent label based detection of waterborne pathogens in microfluidic channels, a project I was involved with during my sabbatical at UCLA last year. Traditionally, fluorescent detection is done using expensive fluorescent microscopes. However, with the advent of millions of pixels available on image sensors, it is now possible to convert smart phones or USB-powered small cameras into computational microscopes. I will describe a fluorescent microscope I built using a USB-powered camera, and results of some detection experiments for Giardia Lamblia, a waterborne pathogen.

10:50 a.m. Refreshments
11:00 a.m. Seminar